DNA Modification Detection Methods

DNA Modification Detection Methods
Author :
Publisher : Humana
Total Pages : 194
Release :
ISBN-10 : 107161228X
ISBN-13 : 9781071612286
Rating : 4/5 (286 Downloads)

Book Synopsis DNA Modification Detection Methods by : Bi-Feng Yuan

Download or read book DNA Modification Detection Methods written by Bi-Feng Yuan and published by Humana. This book was released on 2021-07-07 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years. This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.


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