New Computational Tools for Sample Purification and Early-Stage Data Processing in High-Resolution Cryo-Electron Microscopy

Download or read book New Computational Tools for Sample Purification and Early-Stage Data Processing in High-Resolution Cryo-Electron Microscopy written by Lukas Schulte and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Large macromolecular protein complexes play an important role in the quest of un- derstanding life at a molecular level. For this endeavor, the highly-resolved 3D structure of a large complex and its changes, which are called conformational dy- namics, are of high interest due to their close relation to biological function. A pop- ular method to gain access to this information is cryo-electron microscopy. Here, biological samples are vitrified in their native buffer conditions and then im- aged in a transmission electron microscope. The large amount of recorded noisy images, called microgra...