Nitric Oxide Synthase Genetic Regulation and Contribution to Aminoglycoside Sensitivity in Staphylococcus Aureus

Download or read book Nitric Oxide Synthase Genetic Regulation and Contribution to Aminoglycoside Sensitivity in Staphylococcus Aureus written by Jessica Nicole Brandwein and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: strain-dependent, as nos expression remained unchanged in an agr mutant in a USA300 strain, which has higher Agr activity compared to UAMS-1 (USA200 strain). Furthermore, nos expression was higher in USA300 relative to UAMS-1 during late-exponential low-oxygen growth, when nos expression is maximal. Global regulators Rex and MgrA were also implicated as negative regulators of nos expression in UAMS-1. Finally, mapping of the nos transcriptional start site revealed that the nos transcript may be subject to processing or may be a leaderless mRNA. Collectively, these results provide new insight into the transcriptomic response of S. aureus to gentamicin treatment as well as factors that control nos expression. Observed strain-dependent differences reinforce the importance of studying bacterial NOS regulation and function at both the strain and species level.