Subcellular Fractionation

Subcellular Fractionation
Author :
Publisher :
Total Pages : 0
Release :
ISBN-10 : 1621820424
ISBN-13 : 9781621820420
Rating : 4/5 (420 Downloads)

Book Synopsis Subcellular Fractionation by : Paul R. Pryor

Download or read book Subcellular Fractionation written by Paul R. Pryor and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.


Subcellular Fractionation Related Books

Subcellular Fractionation
Language: en
Pages: 0
Authors: Paul R. Pryor
Categories: Cell fractionation
Type: BOOK - Published: 2015 - Publisher:

DOWNLOAD EBOOK

Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the task
Subcellular Fractionation
Language: en
Pages: 360
Authors: John M. Graham
Categories: Medical
Type: BOOK - Published: 1997 - Publisher: Oxford University Press

DOWNLOAD EBOOK

In the fast changing world of cell biology it is often required that particular membranes or subcellular organisms be isolated from the structure for investigat
Subcellular Proteomics
Language: en
Pages: 397
Authors: Eric Bertrand
Categories: Science
Type: BOOK - Published: 2007-08-29 - Publisher: Springer Science & Business Media

DOWNLOAD EBOOK

This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organizat
Adipose Tissue Protocols
Language: en
Pages: 332
Authors: GĂ©rard Ailhaud
Categories: Science
Type: BOOK - Published: 2008-02-03 - Publisher: Springer Science & Business Media

DOWNLOAD EBOOK

Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduct
Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate
Language: en
Pages: 408
Authors:
Categories: Science
Type: BOOK - Published: 2013-10-30 - Publisher: Academic Press

DOWNLOAD EBOOK

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-a