The Regulation of Nitric Oxide Levels in Staphylococcus Aureus by Nitric Oxide Synthase and Nitric Oxide Reductase, and Their Effects on Physiology

Download or read book The Regulation of Nitric Oxide Levels in Staphylococcus Aureus by Nitric Oxide Synthase and Nitric Oxide Reductase, and Their Effects on Physiology written by April Melanie Sapp and published by . This book was released on 2014 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: NO-challenged wild-type, nor, hmp, and nor hmp mutant biofilms suggested that hmp may have a greater contribution to intracellular NO detoxification, but nor has a significant impact on intracellular NO levels. As expected, an hmp mutant displayed an impaired ability to initiate growth under nitrosative stress conditions. However, nor mutant growth was comparable to the wild-type strain. Membrane potential and respiratory activity assays indicated that saNOR contributes to membrane potential and respiration under low-oxygen nitrosative stress conditions. Additionally, nor expression was found to be dependent on the SrrAB two-component system that regulates expression of genes involved in respiration and nitrosative stress. In summary, saNOS has an important role in S. aureus physiology and virulence and saNOR contributes to energy generation via respiration of NO during nitrosative stress, possibly conferring an advantage to nor+ strains in vivo.