An in Vitro Comparison of the Osteogenic Potential of Equine Stem Cell Populations and Subpopulations from Multiple Tissue Sources

Download or read book An in Vitro Comparison of the Osteogenic Potential of Equine Stem Cell Populations and Subpopulations from Multiple Tissue Sources written by Catherine L. Radtke and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: An in vitro comparison of the osteogenic potential of equine stem cell populations and subpopulations from multiple tissue sources was made to identify the ideal equine donor tissue as a source of MSCs to promote bone healing. Equine muscle tissue- and periosteal tissue-derived cells where characterized as mesenchymal stem cells (MSCs) and their proliferation capacity and osteogenic potential was assessed in comparison with bone marrow- and adipose tissue-derived MSCs. Cells were isolated from skeletal muscle, periosteal, and adipose tissues, and sternal bone marrow aspirates. Morphology, adherence to plastic, trilineage differentiation, and detection of stem cell surface markers CD44 and CD90 were used to characterize cells as MSCs. Osteogenic potential of MSCs was measured by osteocalcin gene expression. Mesenchymal stromal cell cultures were counted at 24, 48, 72, and 96 hours to determine tissue-specific MSC proliferative capacity. Muscle MSCs (MMSCs), periosteum MSCs (PMSCs), and adipose MSCs (AMSCs) proliferated significantly faster than did bone marrow MSCs (BMSCs) at 72 and 96 hours. Non-equilibrium gravitational field-flow fractionation (GrFFF) was validated as a method for sorting MSCs from four donor sources (muscle, periosteum, bone marrow, and adipose tissue) into subpopulations. Aliquots of MSCs from each tissue source were consistently separated into 6 fractions by continuous flow (GrFFF proprietary system) and these fractions remained viable for use in further assays. Absorbencies (OD) were compared, and trilineage assays performed. Statistical analysis of the fraction absorbencies (OD) revealed a P-value of