Manipulating the Mouse Embryo

Manipulating the Mouse Embryo
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Publisher :
Total Pages : 0
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ISBN-10 : 1936113015
ISBN-13 : 9781936113019
Rating : 4/5 (019 Downloads)

Book Synopsis Manipulating the Mouse Embryo by : Richard Behringer

Download or read book Manipulating the Mouse Embryo written by Richard Behringer and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.


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