Protein Phosphorylation

Download or read book Protein Phosphorylation written by D. Grahame Hardie and published by IRL Press. This book was released on 1993 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reversible phosphorylation is one of the major mechanisms by which protein activity is controlled in all eukaryotic cells. Networks of protein kinases and phosphatases, which catalyze phosphorylation and dephosphorylation respectively, are responsible for switching between different pathways of cell division, development, or differentiation in response to hormones or growth factors. This book provides a comprehensive description of current methods for studying protein phosphorylation and the protein kinases and phosphatases which catalyze it. It includes detailed protocols for studying phosphorylation in intact cells, approaches using enzyme inhibitors, purification of serine/threonine-specific protein kinases/phosphatases, analysis of kinase/phosphatase specificity, and cloning relevant cDNAs. Protein Phosphorylation: A Practical Approach will be of great value to all researchers in the fields of intracellular and intercellular signalling, including biochemists, molecular biologists and pharmacologists.