Fertilization of Salmonid Eggs with Cryopreserved Sperm

Download or read book Fertilization of Salmonid Eggs with Cryopreserved Sperm written by A. G. Ott and published by . This book was released on 1970 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: Numerous fishery researchers have attempted to preserve viable fish sperm, but only three investigations were successful in the fertilization of fresh eggs with frozen and thawed sperm: Blaxter (1953); Hoyle and Idler (1968); and Graybill and Horton (1969). Significant advances reported in this thesis were: the development of suitable life extenders for semen of steelhead trout (Salmo gairdneri), and coho (Oncorhynchus kisutch) and Chinook (0. tshawytscha) salmon; the selection of a life protector composed of 8.0% dimethyl. sulfoxide; the verification that the best rate of freezing semen was -30 C/mm, arid that the best method of thawing frozen sperm was in a 4 C water bath; and the determination that no equilibration time was necessary when fresh semen was diluted with extenders and protectors. Application of the above techniques led to the successful cryo-preservation of male gametes of steelhead trout and Chinook and coho salmon in liquid nitrogen ( -196 C). Frozen and thawed sperm was mixed with fresh eggs and resulted in maximum fertilizations of 59.1% for steelhead trout, 38.4% for spring Chinook salmon, and 79.2% for coho salmon. Details of the chemical constituents of the extenders and protectors tested are appended.