Gene Targeting and Embryonic Stem Cells

Download or read book Gene Targeting and Embryonic Stem Cells written by Jim Mcwhir and published by Garland Science. This book was released on 2004-05-01 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.