Transcriptome Analysis of Common Bean (Phaselous Vulgaris) in Response to Infection by Bacterial Bight Pathogen Xanthomonas Axonopodis Pv. Phaseoli

Download or read book Transcriptome Analysis of Common Bean (Phaselous Vulgaris) in Response to Infection by Bacterial Bight Pathogen Xanthomonas Axonopodis Pv. Phaseoli written by Laura Cox and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the most devastating diseases affecting common bean (Phaseolus vulgaris L.) production is common bacterial blight (CBB) caused by the bacterium Xanthomonas axonopodis pv. phaseoli (Xap). Breeding for resistant cultivars represents the best strategy for CBB immunity, but the molecular mechanisms underlying the interaction between P. vulgaris and CBB are little understood. A transcriptome analysis was undertaken to elucidate this plant-pathogen interaction in a CBB-resistant recombinant inbred line (RIL) and a CBB-susceptible RIL. A total of 800 differentially expressed genes were identified following Xap inoculation, with the majority upregulated in the CBB-resistant RIL 48 hours after inoculation. Upregulated genes included isoflavonoid biosynthesis genes. A metabolite analysis revealed that the isoflavonoids daidzein and genistein, and the phytoalexins coumestrol and phaseollinisoflavan accumulated in the CBB-resistant RIL. These findings are important for targeted breeding approaches aiming to enrich these phytoalexins in P. vulgaris as a strategy to enhance resistance to CBB.