UV and Chemical Crosslinking Mass Spectrometry for the Analysis of Protein-nucleic Acid Interactions

Download or read book UV and Chemical Crosslinking Mass Spectrometry for the Analysis of Protein-nucleic Acid Interactions written by Luisa Welp and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-nucleic acid interactions are a key part of essential cellular processes and their disturbance often results in the development of diseases. Crosslinking mass spectrometry (XL-MS)-based approaches have become increasingly popular for the proteome-wide discovery of nucleic acid-binding proteins. The basic principle of the methodology relies on covalent attachment of proteins and binding nucleic acids by crosslinking, which makes the heteroconjugates amenable to MS analysis. Building up on comprehensive nucleic acid-binding protein inventories, there is an increasing demand for higher...